Tissue:Article Title: Mediation of antitumor activity by AZD4820 oncolytic vaccinia virus encoding IL-12
Article Snippet: .. Cell lines and culture conditions Cell line Tissue origin Source Growth medium SW780 Bladder ATCC RPMI 1640 + 10% FBS T24 Bladder ATCC McCoys 5A + 10% FBS NCI-H292 NSCLC ATCC DMEM + 10% FBS MDA-MB-435 Breast ATCC RPMI 1640 + 10% FBS HUH-7 HCC ATCC RPMI 1640 + 10% FBS A375 Melanoma ATCC DMEM + 10% FBS OVCAR3 Ovary ATCC RPMI 1640 + 10% FBS HEP3B HCC ATCC DMEM + 10% FBS PANC-1 Pancreas ATCC DMEM + 10% FBS RPMI7951 Melanoma ATCC DMEM + 10% FBS HCC38 Breast ATCC RPMI 1640 + 10% FBS HUPT3 Pancreas ATCC DMEM + 10% FBS A549 NSCLC ATCC F12K + 10% FBS TCCSUP Bladder ATCC DMEM + 10% FBS HCT-116 CRC ATCC RPMI 1640 + 10% FBS SK-MEL-5 Melanoma ATCC DMEM + 10% FBS ACHN Kidney ATCC DMEM + 10% FBS SW1417 CRC ATCC RPMI 1640 + 10% FBS DAN-G Pancreas ATCC DMEM + 10% FBS OVCAR4 Ovary ATCC RPMI 1640 + 10% FBS A498 Kidney ATCC DMEM + 10% FBS BT20 Breast ATCC DMEM + 10% FBS PC3 Prostate ATCC F12K + 10% FBS KATO-III Gastric ATCC IMDM + 20% FBS NCI-N87 Gastric ATCC RPMI 1640 + 10% FBS WIDR CRC ATCC DMEM + 10% FBS SNU5 Gastric ATCC IMDM + 20% FBS Cell line Tissue origin Source Growth medium NCI-H460 NSCLC ATCC RPMI 1640 + 10% FBS LNCAP Prostate ATCC RPMI 1640 + 10% FBS MM1R Myeloma ATCC RPMI 1640 + 10% FBS HEK–Blue IL-12SEAP Human embryonic kidney InvivoGen DMEM, 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, 100 μg/mL Normocin Growth conditions were 5% CO2, 37°C. .. ATCC, American Type Tissue Culture Collection; CRC, colorectal cancer; DMEM, Dulbecco modified Eagle medium; FBS, fetal bovine serum; HCC, hepatocellular cancer; NSCLC, non– small-cell lung cancer; SEAP, secreted alkaline phosphatase.
Multiple Displacement Amplification:Article Title: Mediation of antitumor activity by AZD4820 oncolytic vaccinia virus encoding IL-12
Article Snippet: .. Cell lines and culture conditions Cell line Tissue origin Source Growth medium SW780 Bladder ATCC RPMI 1640 + 10% FBS T24 Bladder ATCC McCoys 5A + 10% FBS NCI-H292 NSCLC ATCC DMEM + 10% FBS MDA-MB-435 Breast ATCC RPMI 1640 + 10% FBS HUH-7 HCC ATCC RPMI 1640 + 10% FBS A375 Melanoma ATCC DMEM + 10% FBS OVCAR3 Ovary ATCC RPMI 1640 + 10% FBS HEP3B HCC ATCC DMEM + 10% FBS PANC-1 Pancreas ATCC DMEM + 10% FBS RPMI7951 Melanoma ATCC DMEM + 10% FBS HCC38 Breast ATCC RPMI 1640 + 10% FBS HUPT3 Pancreas ATCC DMEM + 10% FBS A549 NSCLC ATCC F12K + 10% FBS TCCSUP Bladder ATCC DMEM + 10% FBS HCT-116 CRC ATCC RPMI 1640 + 10% FBS SK-MEL-5 Melanoma ATCC DMEM + 10% FBS ACHN Kidney ATCC DMEM + 10% FBS SW1417 CRC ATCC RPMI 1640 + 10% FBS DAN-G Pancreas ATCC DMEM + 10% FBS OVCAR4 Ovary ATCC RPMI 1640 + 10% FBS A498 Kidney ATCC DMEM + 10% FBS BT20 Breast ATCC DMEM + 10% FBS PC3 Prostate ATCC F12K + 10% FBS KATO-III Gastric ATCC IMDM + 20% FBS NCI-N87 Gastric ATCC RPMI 1640 + 10% FBS WIDR CRC ATCC DMEM + 10% FBS SNU5 Gastric ATCC IMDM + 20% FBS Cell line Tissue origin Source Growth medium NCI-H460 NSCLC ATCC RPMI 1640 + 10% FBS LNCAP Prostate ATCC RPMI 1640 + 10% FBS MM1R Myeloma ATCC RPMI 1640 + 10% FBS HEK–Blue IL-12SEAP Human embryonic kidney InvivoGen DMEM, 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, 100 μg/mL Normocin Growth conditions were 5% CO2, 37°C. .. ATCC, American Type Tissue Culture Collection; CRC, colorectal cancer; DMEM, Dulbecco modified Eagle medium; FBS, fetal bovine serum; HCC, hepatocellular cancer; NSCLC, non– small-cell lung cancer; SEAP, secreted alkaline phosphatase.
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